Puffin-D

Puffin-D Prediction

Base-resolution transcription initiation prediction for sequences of 100 kb or more.

SeqStr format:
<sequence_name>[reference_genome]chromosome:coordinate-coordinate strand,@chromosome coordinate reference_alleles alternative_alleles;raw sequence;
Example:
<mutant>[hg38]chr7:5480600-5580600 -, @chr7 5530626 TATA GCGC;TTAAccggGGNaa
Use line breaks to add and compare multiple sequences
Puffin-D requires at least 100kbp. Longer sequences will be truncated at both ends and only keep the central 100kbp